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Involvement of Lipoxygenase-dependent Production of Fatty Acid Hydroperoxides in the Development of the Hypersensitive Cell Death induced by Cryptoge…

1999

Lipid peroxidation was investigated in relation with the hypersensitive reaction in cryptogein-elicited tobacco leaves. A massive production of free polyunsaturated fatty acid (PUFA) hydroperoxides dependent on a 9-lipoxygenase (LOX) activity was characterized during the development of leaf necrosis. The process occurred after a lag phase of 12 h, was accompanied by the concomitant increase of 9-LOX activity, and preceded by a transient accumulation of LOX transcripts. Free radical-mediated lipid peroxidation represented 10% of the process. Inhibition and activation of the LOX pathway was shown to inhibit or to activate cell death, and evidence was provided that fatty acid hydroperoxides ar…

feuille0106 biological sciencesLipid PeroxidesProgrammed cell deathNecrosishypersensibilitéréaction hsLipoxygenasenicotiana tabacumBiology01 natural sciencesBiochemistryFungal ProteinsLipid peroxidation03 medical and health scienceschemistry.chemical_compoundLipoxygenasecryptogénieTobaccomedicineperoxyde[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM]LipaseMolecular BiologyComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesCell Deathacide grasAlgal Proteinsfood and beveragesCell BiologyRespiratory bursttabacChloroplastPlants ToxicchemistryBiochemistrybiology.proteinlipids (amino acids peptides and proteins)peroxydationmedicine.symptomSignal Transduction010606 plant biology & botanyPolyunsaturated fatty acidJournal of Biological Chemistry
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